HTB-38 HT-29 人結(jié)腸癌細(xì)胞

ATCC® Number:  HTB-38™

Designations:  HT-29

Depositors:

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: colon

Disease: colorectal adenocarcinoma

Cellular Products: secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:  Isolation date: 1964

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: human adrenergic alpha2A [23560]

urokinase receptor (u-PAR)

vitamin D (moderate expression)

urokinase receptor (u-PAR); vitamin D (moderate expression)

Virus Susceptibility: human immunodeficiency virus (HIV, LAV)

Tumorigenic: Yes

Oncogene: myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -

Antigen Expression: Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 11,12

D16S539: 11,12

D5S818: 11,12

D7S820: 10

THO1: 6,9

TPOX: 8,9

vWA: 17,19

Cytogenetic Analysis: modal number = 71; range = 68 to 72.

The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.

Isoenzymes:  AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1-2

PGM3, 1-2

Age:  44 years adult

Gender:  female

Ethnicity:  Caucasian

Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes. The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected.

There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

derivative:ATCC CCL-218

purified DNA:ATCC HTB-38D

HTB-38 HT-29 人結(jié)腸癌細(xì)胞

MLE-12 小鼠肺上皮細(xì)胞  

saos-2 人成骨肉瘤細(xì)胞

MA104 非洲綠猴胚胎腎上皮細(xì)胞

ball-1 細(xì)胞

nci-h226 人肺鱗癌細(xì)胞

MCA38 小鼠結(jié)腸癌細(xì)胞

LTPA 小鼠胰腺癌細(xì)胞

NCI-H23 人非小細(xì)胞肺癌細(xì)胞

293.00     人胚腎細(xì)胞

293FT    人胚腎細(xì)胞-用于慢病毒包裝

293TN    人胚腎細(xì)胞--用于慢病毒包裝

293AV    人胚腎細(xì)胞-用于腺病毒包裝

293T    人胚腎細(xì)胞

208F    大鼠成纖維細(xì)胞

786-O    人腎透明細(xì)胞腺癌細(xì)胞

A172    人腦膠質(zhì)母細(xì)胞瘤細(xì)胞

A549    人肺癌細(xì)胞 細(xì)胞系購買

AGS    人胃癌細(xì)胞

BEL7402 人肝癌細(xì)胞系

AZ-521    人胃癌細(xì)胞

B16F0    小鼠黑色素瘤細(xì)胞

Bel-7402    人肝癌細(xì)胞

Bel-7404    人肝癌細(xì)胞

BGC-823    人胃癌細(xì)胞

C6    大鼠腦膠質(zhì)瘤細(xì)胞

SW480    人結(jié)腸腺癌細(xì)胞

CaCo-2    人結(jié)腸癌細(xì)胞

Cho-K1    倉鼠卵巢細(xì)胞亞株

COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞

GES-1    人胃上皮細(xì)胞

HCT-8    人回盲腸癌細(xì)胞

HeLa

Hepa1-6    小鼠肝癌細(xì)胞

HepG2    人肝癌細(xì)胞

hFOB1.19    人永生化成骨細(xì)胞

HGC-27    人胃癌細(xì)胞

Hs 746T    人胃癌細(xì)胞

HT-29    人結(jié)腸癌細(xì)胞

KB    人口腔表皮樣癌細(xì)胞

LoVo    人結(jié)腸癌細(xì)胞

LLC-PK1    豬腎近曲小管上皮細(xì)胞

MCF-10A    人正常乳腺上皮細(xì)胞

MCF-7    人乳腺癌細(xì)胞

MDA-MB-231    人乳腺癌細(xì)胞

MGC80-3    人胃癌細(xì)胞

MKN-45    人胃癌細(xì)胞

NCI-H460    人大細(xì)胞肺癌細(xì)胞

NIH-3T3    小鼠成纖維細(xì)胞

NUGC-3    人胃癌細(xì)胞

Raw264-7    小鼠單核細(xì)胞白血病細(xì)胞

SGC-7901    人胃癌細(xì)胞

SMMC-7721    人肝癌細(xì)胞

SNU-638    人胃癌細(xì)胞

SNU-739    人肝癌細(xì)胞

Tca8113    人舌鱗癌細(xì)胞    細(xì)胞系購買

U251    人腦膠質(zhì)瘤細(xì)胞

U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞

U-87 MG    人腦星形膠質(zhì)母細(xì)胞瘤

BT-474    人乳腺導(dǎo)管癌細(xì)胞

PANC-1    人胰腺癌細(xì)胞

HEK293    人胚腎細(xì)胞

Hep3B    人肝癌細(xì)胞

HL-7702    人正常肝細(xì)胞

Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞

WEHI-231    小鼠B淋巴細(xì)胞

OPM-2    人骨髓瘤細(xì)胞

NCI-H929    人骨髓瘤細(xì)胞

U266    人骨髓瘤細(xì)胞

EJ    人膀胱癌細(xì)胞

HOC    大鼠肝卵圓細(xì)胞

RWPE-1    人正常前列腺上皮細(xì)胞

CAL 27    人舌鱗癌細(xì)胞

Calu-3    人肺腺癌細(xì)胞

Eca-109    人食管癌細(xì)胞

DLD-1    人結(jié)直腸腺癌上皮細(xì)胞

5637.00     人膀胱癌細(xì)胞

SK-OV-3    人卵巢癌細(xì)胞

GBC-SD    人膽囊癌細(xì)胞

BEL7402 人肝癌細(xì)胞系

SW620    人結(jié)腸癌細(xì)胞

MRC-5    人胚肺成纖維細(xì)胞     細(xì)胞系購買

PC9    人肺癌細(xì)胞

PC14    人肺癌細(xì)胞

BxPC    人原位胰腺腺癌細(xì)胞

PK（15）    豬腎上皮細(xì)胞

MDCK    狗腎上皮細(xì)胞

3D4/21    豬肺泡巨噬細(xì)胞

MHCC-97L    人肝癌細(xì)胞（低轉(zhuǎn)移）

MHCC-97H    人肝癌細(xì)胞系（高轉(zhuǎn)移）

MiaCaPa-2    人胰腺癌細(xì)胞

HN13    口腔鱗狀細(xì)胞癌細(xì)胞

Bend3    小鼠腦微血管內(nèi)皮細(xì)胞

QBC939    人膽管癌細(xì)胞

Ishikawa    人子宮內(nèi)膜癌細(xì)胞

A498    人腎癌細(xì)胞

143B

WEHI-231    小鼠B淋巴細(xì)胞

BN

P9R    小鼠骨髓基質(zhì)細(xì)胞

P9    小鼠骨髓基質(zhì)細(xì)胞

F98    大鼠腦膠質(zhì)瘤細(xì)胞

NCIH1299    人非小細(xì)胞肺癌細(xì)胞

NCIH1650-M3    人非小細(xì)胞肺癌細(xì)胞

293LVT    人胚腎細(xì)胞--用于慢病毒包裝

EC9706    人食管癌細(xì)胞

CNE    人鼻咽癌細(xì)胞

H322    人非小細(xì)胞肺癌細(xì)胞

Panc O2

S8    BEL7402 人肝癌細(xì)胞系

R5    細(xì)胞系購買

Pig MSC    豬骨髓間質(zhì)干細(xì)胞

HCAEC    人冠狀動(dòng)脈內(nèi)皮細(xì)胞

marc-145    猴胚胎腎上皮細(xì)胞

3D4/21    豬肺泡巨噬細(xì)胞

PK（15）    豬腎上皮細(xì)胞

MADB-106    大鼠乳腺癌細(xì)胞

Sf9    昆蟲卵巢細(xì)胞

DADY

HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細(xì)胞

PC14    人肺癌細(xì)胞

PC9    人肺癌細(xì)胞

Eca-109    人食管癌細(xì)胞

CAL 27    人舌鱗癌細(xì)胞

HOC    肝卵圓細(xì)胞

EJ    人膀胱癌細(xì)胞

U266    人骨髓瘤細(xì)胞

293-mTLR5    人胚腎細(xì)胞

Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞

HL-7702    人正常肝細(xì)胞

Hep3B    人肝癌細(xì)胞

U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞

Tca-8113    人舌鱗癌細(xì)胞

SNU-739    人肝癌細(xì)胞

SNU-638    人胃癌細(xì)胞

NUGC-3    人胃癌細(xì)胞

NIH-3T3    小鼠成纖維細(xì)胞

MKN-45    人胃癌細(xì)胞

LLC-PK1    豬腎近曲小管上皮細(xì)胞

Hs 746T    人胃癌細(xì)胞

Hepa1-6    小鼠肝癌細(xì)胞

HCT-8    人回盲腸癌細(xì)胞

BEL7402 人肝癌細(xì)胞系

HaCaT    人永生化表皮細(xì)胞

GES-1    人胃上皮細(xì)胞

B16-F0    小鼠黑色素瘤細(xì)胞

AZ-521    人胃癌細(xì)胞

786-O    人腎透明細(xì)胞腺癌細(xì)胞

208F    大鼠成纖維細(xì)胞

293TN    人胚腎細(xì)胞--用于慢病毒包裝

293FT    人胚腎細(xì)胞-用于慢病毒包裝

GP-293    人胚腎細(xì)胞

293-L.P.    人胚腎細(xì)胞