CRL-1596 RAMOS(RA 1) 人B淋巴細(xì)胞瘤

ATCC® Number: CRL-1596™ Price: $256.00

Designations: Ramos （RA 1）

Depositors: BC Giovanella

Isotype: IgM; lambda light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens （human）

Morphology: lymphoblast

Source: Disease: Burkitt's lymphoma （American）

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin （surface and secreted） [1093] [22747]

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CRL-1596 RAMOS(RA 1) 人B淋巴細(xì)胞瘤

Applications: transfection host （Nucleofection technology from Lonza）

Receptors: interleukin 4 （IL-4）; low affinity IgE （CD23） [23468]

Tumorigenic: Yes

Antigen Expression: CD23 + [23468]

DNA Profile （STR）: Amelogenin: X

CSF1PO: 10,11

D13S317: 13,14

D16S539: 10,13

D5S818: 7,12

D7S820: 11

THO1: 7,9.3

TPOX: 8,9

vWA: 15,16

Age: 3 years

Gender: male

Ethnicity: Caucasian

Comments: The cells are negative for Epstein-Barr virus. [22747]

Both the parental line （ATCC CRL-1596）and the derivative cell line Ramos.2G6.4C10 （ATCC CRL-1923） have about 1500 IL-4 binding sites per cell. [23389]

The cells are reported to secrete IgM （lambda light chain）. [1093]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days （depending on cell density）

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

derivative:ATCC CRL-1923

References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

22747: Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

23389: Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

23468: Rousset F, et al. Regulation of Fc receptor for IgE （CD23） and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726

26047: Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896

32796: Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082

CRL-1596 RAMOS(RA 1) 人B淋巴細(xì)胞瘤