CRL-2132 DSL-6A/C1 大鼠胰腺癌細(xì)胞， ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CRL-2132 DSL-6A/C1 大鼠胰腺癌細(xì)胞

ATCC® Number: CRL-2132™ Price: $438.00

Designations: DSL-6A/C1

Depositors: OS Pettengill, D Longnecker

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus （rat）

Morphology: epithelial

Source: Organ: pancreas

Strain: Lewis

Disease: pancreatic carcinoma, azaserine induced

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Note: These cells are distributed subject to the following: 1.） This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.） Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. （603） 646-3675. 3.） In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

Tumorigenic: Yes

Gender: male

Comments: DSL-6A/C1 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.

The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat （DSL-101-79） that was given azaserine intraperitoneally.

The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.

The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting.

The DSL-6A/C1 cell line expresses the ductal marker cystic fibrosis transmembrane regulator （CFTR）.

Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%

Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline （D-PBS） or 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

5.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23188: Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218